Generator
LacI:CspC
Part:BBa_K538204
Designed by: Bas Stringer Group: iGEM11_Amsterdam (2011-09-08)
pLacI + RBS + CspC
This part was submitted by team [http://2011.igem.org/Team:Amsterdam Amsterdam 2011] as part of their project: icE. coli. Its purpose is to express the CspC protein from Polaribacter irgensii (BBa_K538004) under control of the Lac operon's promoter, pLacI (BBa_R0010). E. coli expressing this protein experience a drastic increase in their ability to survive freezing and thawing. This Brick's designers intend to use it to culture 'normal' E. coli faster at temperatures below its optimum (37°C), possibly even facilitating growth below the minimal growth temperature of 7°C.
Refer to the main page of the coding region (CspC: BBa_K538004) for more information on how CspC facilitates cold resistance in E. coli.
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
//classic/generator/prc
//function/regulation/transcriptional
//regulation/negative
//function/regulation/transcriptional
//regulation/negative
function | Protein expression |
negative_regulators | LacI |
output | CspC |